Experiment was conducted at the National Horticultural Research Institutes experimental field between July 2003 and January 2005 to determine the effect of some cultural practices on the populations of plant parasitic nematodes infestation, and on some growth and yield attributes of plantain cv. Agbagba. The treatments included corm paring+hot water root dip, Titonia diversifolia mulch, and combination of Tithonia mulch and hot water root dip. The untreated plantlets served as the control. The control plots recorded the highest populations of nematodes Meloidogyne incognita, Helicotylenchus multicintus and Radopholus similes, while the lowest populations occurred in plots with the treatment combination of hot water root dip and T. diversifolia mulch. Hot water treatment followed by Tithonia mulch significantly enhanced plant height number of leaves and sucker production. It cause early flowering and fruiting and significantly increased bunch weight. This practice is recommended for plantain producers in organic agriculture production system.